Polymerase Chain Reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor

Tentcheva , Diana
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Gauthier , Laurent
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Jouve , Sandrine
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Canabady-Rochelle , Laetitia
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Dainat , Benjamin
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Cousserans , Francois
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Colin , Marc Edouard
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée; Ball , Brenda V.
        . Plant and Invertebrate Ecology Division; Bergoin , Max
        INRA
         .
         UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée

Polymerase Chain Reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor

2004

Tentcheva , Diana (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Gauthier , Laurent (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Jouve , Sandrine (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Canabady-Rochelle , Laetitia (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Dainat , Benjamin (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Cousserans , Francois (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Colin , Marc Edouard (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée) | Ball , Brenda V. (Rothamsted Research, Herts(Royaume Uni). Plant and Invertebrate Ecology Division) | Bergoin , Max (INRA (France). UMR 1231 UMR INRA / FRE CNRS / Univ. Montpellier 2 : Pathologie Comparée)

We have developed a specific assay for the detection of deformed wing virus (DWV) in Apis mellifera L. and Varroa destructor based on the reverse transcriptase polymerase chain reaction (RT-PCR) technology. Primers were designed from the sequence of a 4700 nucleotides cDNA fragment located in the 3'-end of the DWV genome. This fragment encodes a single open reading frame of 1565 amino acids showing similarity to viral RNA dependent RNA polymerase consensus motif. RT-PCR assays from DWV infected individual mite or bee produced a 395 nucleotide DNA fragment clearly identifiable by agarose gel electrophoresis. The signal in bees having deformed wings was significantly higher than in normal ones. A search for DWV in 40 colonies showed that DWV is broadly distributed in bee colonies and mites. As an average, greater virus prevalence of virus was detected in bees collected in autumn compared to bees collected in spring or during the summer period

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