Systematics of the Dioryctria abietella species group (Lepidoptera: Pyralidae) based on mitochondrial DNA

Roux-Morabito , Geraldine
        INRA
        , Ardon .
         UR 0633 Unité de Recherche Zoologie Forestière; Gillette , Nancy
        . Pacific Southwest Research Station; Roques , Alain
        INRA
        , Ardon .
         UR 0633 Unité de Recherche Zoologie Forestière; Dormont , Laurent
        . UMR 5175, Centre d'Ecologie Fonctionnelle et Evolutive; Stein , John
        . Forest Health Technology Enterprise Team; Sperling , Felix
        . Department Biological Sciences

Systematics of the Dioryctria abietella species group (Lepidoptera: Pyralidae) based on mitochondrial DNA

2008

Roux-Morabito , Geraldine (INRA , Ardon (France). UR 0633 Unité de Recherche Zoologie Forestière) | Gillette , Nancy (US Forest Service, Albany(Etats-Unis). Pacific Southwest Research Station) | Roques , Alain (INRA , Ardon (France). UR 0633 Unité de Recherche Zoologie Forestière) | Dormont , Laurent (Centre National de la Recherche Scientifique, Montpellier(France). UMR 5175, Centre d'Ecologie Fonctionnelle et Evolutive) | Stein , John (US Forest Service, Morgantown(Etats-Unis). Forest Health Technology Enterprise Team) | Sperling , Felix (University of Alberta, Edmonton(Canada). Department Biological Sciences)

Coneworms of the genus Dioryctria Zeller include a number of serious pests of conifer seeds that are notoriously difficult to distinguish as species. We surveyed mitochondrial DNA variation within the abietella species group by sequencing 451 bp of COI and 572 bp of COII genes from 64 individuals of 6 major species in the group. In addition to examining phylogenetic relationships within European members of the group, the study focused on the two most damaging species, D. abietivorella Grote from North America and D. abietella Denis and Schiffermüller from Europe and Asia, which have been considered taxonomically synonymous in the past. In order to detect different levels of divergence, we extensively sampled in seed orchards and natural forests for D. abietella on different hosts. Maximum parsimony and maximum likelihood analyses confirmed the monophyly of the abietella species group and its separation into three clades. The grouping of North American species (Clade A) received strong support in both analyses, whereas relationships between Clade A and the two other European clades were weakly supported. D. simplicella Heinemann could not be unambiguously separated from D. abietella populations. The diverse haplotypes observed in the network analysis conducted with eight populations of polyphagous D. abietella suggested the presence of two distinct lineages in France.

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