Heme binding proteins of Bartonella henselae are required when undergoing oxidative stress during cell and flea invasion
2012
Liu , MAFENG (INRA , Maisons-Alfort (France). UMR 0956 Biologie Moléculaire et Immunologie Parasitaires et Fongiques) | Ferrandez , Yann (Ecole Nationale Vétérinaire d'Alfort, Maisons Alfort(France). Ecole Natl Vet Alfort, INRA Anses UPEC ENVA) | Bouhsira , Emilie (Université de Toulouse, Toulouse(France). Lab Parasitol, INP, ENVT) | Monteil , Martine (INRA , Maisons-Alfort (France). UMR 0956 Biologie Moléculaire et Immunologie Parasitaires et Fongiques) | Franc , Michel (Université de Toulouse, Toulouse(France). Lab Parasitol, INP, ENVT) | Boulouis , Henri-Jean (INRA , Maisons-Alfort (France). UMR 0956 Biologie Moléculaire et Immunologie Parasitaires et Fongiques) | Biville , Francis (INRA , Maisons-Alfort (France). UMR 0956 Biologie Moléculaire et Immunologie Parasitaires et Fongiques)
Bartonella are hemotropic bacteria responsible for emerging zoonoses. These heme auxotroph alphaproteobacteria must import heme for their growth, since they cannot synthesize it. To import exogenous heme, Bartonella genomes encode for a complete heme uptake system enabling transportation of this compound into the cytoplasm and degrading it to release iron. In addition, these bacteria encode for four or five outer membrane heme binding proteins (Hbps). The structural genes of these highly homologous proteins are expressed differently depending on oxygen, temperature and heme concentrations. These proteins were hypothesized as being involved in various cellular processes according to their ability to bind heme and their regulation profile. In this report, we investigated the roles of the four Hbps of Bartonella henselae, responsible for cat scratch disease. We show that Hbps can bind heme in vitro. They are able to enhance the efficiency of heme uptake when co-expressed with a heme transporter in Escherichia coli. Using B. henselae Hbp knockdown mutants, we show that these proteins are involved in defense against the oxidative stress, colonization of human endothelial cell and survival in the flea.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique
