Decolorization of glucose-glycine melanoidin as model color pollutant using ozonation and bacterial treatment
2007
Victorio, J.D. | Buquiran, D.P. | del Rosario, E.J.
Melanoidin was prepared as model color pollutant by heating equimolar amounts of glucose and glycine in sodium carbonate solution for 3h at 121 deg C. After partial purification by Sephadex G-100 chromatography it was ozonated and then used as culture substrate for Bacillus subtilis. Ozone treatment for 15, 30, and 60 min resulted in decolorization (reduction in absorbance at 475 nm) values of 21.92%, 41.10% and 65.75%, respectively; corresponding molecular weight (MW) values were 18.23, 15.14 and 8.66 kDa compared with a MW of 22.79 kDa for raw melanoidin. The 1-h ozonated melanoidin showed further 22.80% decolorization after bacterial treatment for 5 days. The final MW values after combined ozonation and bacterial treatment were 16.24, 13.36, and 7.61 kDa for 15, 30, and 60 min of ozonation, respectively, followed by 5 days of bacterial treatment.
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