Characterization of the growth of Rhizobium trifolii and Sinorhizobium meliloti in different culture media
2013
NISTE, Monica | VIDICAN, Roxana | ROTAR, Ioan | POP, Rodica
Legumes have the ability to form symbiotic interactions with soil bacteria, called rhizobia. Bacteria of the genus Rhizobium are able to convert atmospheric nitrogen into ammonia when compounds are exchanged between the bacteroid and its plant host. The present study describes the characterization of Rhizobium strains isolated from root nodules of Trifolium pratense and Medicago sativa grown in a greenhouse. The main objective of the experiment was to identify which medium is more suitable for the development of different strains of rhizobia. The Rhizobium strains are rod shaped, Gram negative and mucus producing. The rhizobia were identified and isolated using different media yeast extract mannitol agar (YEMA) containing Congo red, and a medium including boron (B), an essential micronutrient. The Petri plates were incubated at 28ºC and inspected three days after the inoculation. The colony morphology was analysed based on type, appearance, transparency, colour and the effectiveness of boron on Rhizobium growth.
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