Development of ELISA for diagnosis of Johneps Disease by 34 kDa protein from Mycobacterium paratuberculosis
2012
Rasool Madani
Early detection of Mycobacterium avium subsp. Paratuberculosis, Johne`s disease agent Mycobacterium avium subsp. Paratuberculosis (MAP) is the causative agent of Johne`s disease or Paratuberculosis (Severe diarrhea) in the ruminants that can have a severe economic damages to animal husbandry industry in the developed as well as developing countries. Due to the zoonotic behavior of the disease and probability of causing inflammatory bowel disease in humanearly diagnosis of the bacterial agent in the dairy product could be of great importance. The purpose of this study was to find a specific antigen with a rapid and accuratecapability of detecting contamination in the early stages which can prevent the emission of contamination and will lead to significant progress in treatment. In this study we used cell wall immunodominant 34kD protein as antigen in ELISA test (1g/ml ) for diagnosis of paratuberclusis . Cut off value was determined at 1.17 by using positive control and negative control serum sample. The 160 serum sample were evaluated by ELISA test and infected samples were determined . This method has diagnostical value in subclinical johne's disease. Keywords:Mycobacterium avium subsp. Paratuberculosis,Immonodominant protein (P34), ELISA
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Agricultural Research and Education Organization