Simultaneous Determination and Mornitoring of Aflatoxin and Ochratoxin A in Food
2013
Park, J.W., Gwangju Regional Korea Food and Drug Administration, Gwangju, Republic of korea | Yoo, M.S., Gwangju Regional Korea Food and Drug Administration, Gwangju, Republic of korea | Kuk, J.H., Gwangju Regional Korea Food and Drug Administration, Gwangju, Republic of korea | Ji, Y.A., Gwangju Regional Korea Food and Drug Administration, Gwangju, Republic of korea | Lee, J.H., Gwangju Regional Korea Food and Drug Administration, Gwangju, Republic of korea
The simultaneous analysis and monitoring of aflatoxin B1, G1, B2, G2 and ochratoxin A in foods were carried out by HPLC with fluorescence detection. The samples were extracted with methanol/water mixture. The extract was centrifuged, diluted with phosphate buffer saline (PBS), filtered, and applied to an immunoaffinity column containing antibodies specific to both aflatoxins and ochratoxin A. After washing the column with PBS and water, the toxins were eluted from the column with methanol, and quantified by HPLC, with a run time of approximately 30 min. The recoveries for aflatoxin B1, G1, B2, G2 and ochratoxin A in foods were 78.4~101.5%, 73.3~102.1%, 81.7~106.7%, 67.0~104.6% and 78.7~120.8%, respectively. The limits of detection of aflatoxins and ochratoxin A ranged from 0.05 to 0.18 μg/kg. According to monitoring result with the established method, aflatoxin B1 and ochratoxin A were found in 13 of 151 domestic commercial foods. The contamination levels were 0.32~1.80 μg/kg for aflatoxin B1 and 0.97 μg/ kg for ochratoxin A. Therefore, this study showed all commercial foods monitored were safe under the Korean standards for aflatoxins and ochratoxin A.
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