Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces
2014
Kim, Y.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, H.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jeon, A.B., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Bae, Y.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Byun, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10. 10. and 10. CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
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