Discrimination of Echinochloa colona (L.) Link from other Echinochloa Species using DNA Barcode
2015
Lee, J., National Academy of Agricultural Science, Wanju, Republic of Korea | Kim, C.S., National Academy of Agricultural Science, Wanju, Republic of Korea | Lee, I.Y., National Academy of Agricultural Science, Wanju, Republic of Korea
Echinochloa colona is one of the most problematic weeds in the paddy fields of the world. In recent years, this species is likely to be introduced in Korea due to global warming, the expansion of international trade including agricultural products, and increasing tourists. We tried to identify the species from Korean Echinochloa crus-galli and E. oryzicola in order to establish the control measures in case of the initial influx. For this study, Echinochloa colona collected from the National Plant Germplasm System, USA were examined and E. crus-galli and E. oryzicola were collected in Korea. It is, however, very difficult to identify for Echinochloa species using morphological characters because of numerous interspecific and intraspecific types found in nature. Thus, we barcoded the species using rbcL, matK, and ITS. All three markers identified E. colona very well from the others. ITS alone may be enough as a DNA barcode for E. colona identification, when considering cost and effectiveness. The barcode sequences were deposited to the National Center for Biotechnology Information database for public use.
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