Chemical Composition, Antioxidant and Cytoprotective Activities of Lotus Receptacle
2015
Hu, W., Huaiyin Normal University, Huaian, China | Wang, G., Nanjing Medical University, Huaian, China | Shen, T., Huaiyin Normal University, Huaian, China | Wang, Y., Jiangsu Academy of Agricultural Sciences, Nanjing, China | Hu, B., Yangzhou University, Yangzhou, China | Wang, X., Huaiyin Normal University, Huaian, China | Wu, L., Huaiyin Normal University, Huaian, China | Li, P., Jiangsu Academy of Agricultural Sciences, Nanjing, China | Ji, L., Huaiyin Normal University, Huaian, China
The nutritional composition (ash, protein, fat, crude fiber, and carbohydrate) and antioxidant activity of lotus receptacles (LRs) were studied. The LRs contained 88.25% carbohydrates, 22.90% crude fiber, 5.79% crude ash, 0.77% crude protein content, and 5.19% crude lipid on a dry weight basis. The ethanolic extract of lotus receptacle (ELR) exerted strong scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl radical (OH), superoxide anion (O 2- ), and hydrogen peroxide (H2O2), as well as high reducing power. ELR pretreatment effectively elevated cell viability and decreased malondialdehyde (MDA) levels in H2O2-treated RAW 264.7 cells. Additionally, ELR had high phenolic and flavonoid contents with values of 318.15 mg gallic acid equivalent/g and 239.06 mg rutin equivalent/g, respectively. Moreover, the bioactivity-guided study of ELR resulted in the isolation and identification of quercetin-3-O- beta-D-glucopyranoside (QOG), a dietary flavonoid. QOG exhibited strong DPPH free radical scavenging activity, with an IC50 value of 24.26 microM; docking studies revealed that QOC formed one hydrogen bond with LYS263 in the active site, suggesting it is, in part, responsible for the free activity of LR.
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