Evaluation of Pea (Pisum sativum L.) Genetic Resources for Sprout and Analysis of Optimum Culture Condition for Pea Sprout
2015
Koo, S.C., NICS, RDA, Miryang, Republic of Korea | Kang, B.K., NICS, RDA, Miryang, Republic of Korea | Kim, H.T., NICS, RDA, Miryang, Republic of Korea | Seo, J.H., NICS, RDA, Miryang, Republic of Korea | Jeong, K.H., NICS, RDA, Wanju, Republic of Korea | Yun, H.T., NICS, RDA, Miryang, Republic of Korea | Oh, I.S., NICS, RDA, Miryang, Republic of Korea | Choi, M.S., NICS, RDA, Miryang, Republic of Korea
Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25 Celsius). However, whole length, hypocotyl length, and sprout yield were highly increased at 23 Celsius compared to 20 and 25 Celsius. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23 Celsius than at 20 and 25 Celsius, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.
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