Application of Trichoderma asperellum fresh culture bioproduct as potential biological control agent of fungal diseases to increase yield of rice (Oryza sativa L.)
2015
Charoenrak, P. | Chamswarng, C.
Dirty panicle, brown spot, narrow brown spot and sheath rot are important constraints for rice production in Thailand. This research sought to evaluate the efficacy of five isolates of Trichoderma asperellum to reduce these fungal diseases and to increase rice field. Experiments were conducted at Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand from June to September, 2014. Rice seeds var. Chai Nat 1 were soaked (Sk) for 24 hr in spore suspension derived from Trichoderma fresh culture bioproduct (semi-cooked broken milled rice colonized with fungus) at 100 g 20/L. Soaked rice seeds were planted in cement circular wells. Rice plants were sprayed (Sp) with spore suspension (100 g - fresh culture 20/L) three times during the growing period. The results revealed that T. asperellum isolate 01-52 and CB-Pin-01 increased plant-height, number of panicles per square meter, 1,000-seed weight and yield. Dirty panicle infected seed, brown spot and narrow brown spot were reduced when compared to untreated control. The highest percentage of rice roots colonized by T. asperellum was obtained from treatment 01-52 and (Sk + Sp). Fresh culture bioproducts of T. asperellum 01-52 and CB-Pin-01 used for seed soaking and plant spraying have the potential to reduce several important rice fungal diseases and to enhance rice yield.
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