Mapping quantitative trait loci for grain zinc and iron concentrations in four doubled haploid populations of rice (Oryza sativa)
2016
Amparado, A. | Descalsota, G.I. | Asilo, M.A.I. | Tesoro, F. | Reinke, R. | Swamy, B.P.M.
Zinc and iron deficiencies are among the most prevailing nutritional disorders in the world affecting a large number of children and women in rice dependent Asian countries. Breeding rice varieties with enhanced levels of grain zinc and iron concentration is considered the most sustainable and cost effective approach to alleviate micronutrient deficiencies. Development of molecular markers for high grain iron and zinc concentrations to be used in marker-assisted selection can hasten breeding of rice varieties with micronutrient-dense grains. With the objective of mapping quantitative trait loci (QTLs) for grain iron and zinc concentration, the authors developed four different doubled haploid rice populations namely DH5, DH8, DH10 and DH23. These populations were field evaluated over two seasons during 2015 DS and 2015 WS in replicated trials at IRRI. The four DH populations were genotyped using 6k SNP chip at Genotyping Service Lab (GSL) of IRRI. Inclusive composite interval mapping revealed a number of QTLs for grain zinc and iron concentration in the four populations. For grain zinc concentration, analysis of DS data revealed two QTLs in DH5, four QTLs in DH8, and a simple QTL in DH10. Further, analysis of WS grain zinc concentration data identified four QTLs in DH5, three QTLs in DH8, one QTL in DH10, and two QTLs in DH23. For grain iron concentration, a single QTL was detected in DHS and two QTLs were identified in DH23 after analysis of WS data. Results underline chromosome intervals responsible for variations in grain zinc and iron concentration.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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