Detection of toxin genes and RAPD analysis of Bacillus cereus isolates from different soil types
2015
Savić, Dejana (Military Medical Academy, Belgrade (Serbia). Institute of Microbiology) | Jošić, Dragana (Institute of Soil Science, Belgrade (Serbia). Department of Microbiology, Genetic laboratory) | Ristanović, Elizabeta (Military Medical Academy, Belgrade (Serbia). Institute of Microbiology) | Pivić, Radmila (Institute of Soil Science, Belgrade (Serbia). Department for Soil Amelioration and Erosion) | Stanojković-Sebić, Aleksandra (Institute of Soil Science, Belgrade (Serbia). Department for Soil Amelioration and Erosion) | Zorica, Lepšanović (Military Medical Academy, Belgrade (Serbia). Institute of Epidemiology)
The aim of this study was to detect genes for enterotoxins (hbla, entFM and bceT) and for emetic toxin (cer), to determine antibiotic resistance, and to estimate intraspecies diversity in B. cereus isolates by RAPD analysis. B. cereus was identified in 12 out of 117 indigenous Bacillus spp. using the classical microbiological methods and PCR. All isolates were resistant to penicillin and ampicillin, two to tetracyclin and four to trimethoprim-sulphamethoxazole. Also, all isolates produced inducible penicillinases and β-lactamase. Toxin genes were detected with PCR. EntFM and cer genes were present in all isolates, hbla in all, but two, and bceT in none. RAPD analysis was performed with four different primers, two of them designed for this study. The intraspecies diversity revealed 10 different patterns at the 90% similarity level. Two separate clusters were formed regardless of a soil type or utilization. The detection of genes encoding toxins in all B. cereus isolates indicated these bacteria as potentially pathogenic and seriously for human health. Regardless of a soil type or utilization, the RAPD analysis showed high intraspecies heterogeneity in B. cereus isolates. To the best of our knowledge, this is the first study to analyse the presence of entero- and emetic toxin genes and genetic heterogeneity in B. cereus isolates from different soil types and different soil utilization in Serbia.
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