Antibacterial Activity of Cordyceps militaris Waste Product Extract against Paenibacillus larvae
2016
Kim, H.K., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea | Kang, A.R., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea | Lee, M.L., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea | Lee, M.Y., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea | Kang, E.J., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea | Choi, Y.S., National Institute of Agricultural Sciences, R.D.A, Wanju, Republic of Korea
American foulbrood (AFB) is the most serious and dangerous bacterial disease of honeybee brood caused by the spore forming bacterium Paenibacillus larvae. The aim of the present work was to evaluate the antimicrobial effect of a waste product of Cordyceps militaris extract against P. larvae and its potential for the control of AFB. For the test, the waste product of C. militaris was extracted with methanol and partitioned. And then, the fractions were tested for antimicrobial activity against P. larvae by the radial diffusion assay. The result showed that the portions of the ethyl acetate, and water-saturated n-butanol presented inhibitory capacity against strains of P. larvae, whereas the portions of n-hexane and chloroform were presented no inhibitory capacity against P. larvae. One way ANOVA tests for radial diffusion assay data, indicated significant differences. The results demonstrated that waste product of C. militaris possess beneficial properties for honey bee health and this property could be attributed to the kind and percentage of the main components of waste product of C. militaris portions.
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