Monitoring of Malachite Green in Freshwater Fish using LC-MS/MS
2016
Choi, H., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Yuk, D.H., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Park, Y.A., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Jung, B.K., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Hong, M.S., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Yoon, Y.T., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Yi, H.J., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Kim, Y.C., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Park, S.K., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Kim, M.S., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea | Jung, K., Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of Korea
Malachite green was measured in 200 freshwater fish collected from local markets in Seoul using HPLC-DAD and LC-MS/MS. LC-MS/MS method was validated by linearity, accuracy, precision and limits of detection and quantification according to the CODEX's recommendation and HPLC-DAD method was applied according to the Food Code. Malachite green levels above the quantification limit of the LC-MS/MS were determined 18.5% (37) but just 1 fish was shown to contain malachite green by HPLC-DAD. Of 83 domestic fish, 21 fish were detected malachite green (25.3%). Of 117 fish from China, just 16 fish were detected malachite green (13.4%). In detection rate by species carp (35.0%), Crucian carp (30.4%), cat fish (28.0%), Korean bull head (23.8%), snake head (20.0%), eel (10.5%) and loach (7.8%) were in order. Especially, fish collected at summer were shown to contain malachite green frequently; the detection rate was 54.8%.
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