Proliferation, Accumulation of Polyphenols, and Antioxidant Activities of Callus from the ‘Anji Baicha’ Cultivar of Tea [Camellia sinensis (L.) O. Ktze.]
2017
Liu, M., Minzu University of China, Beijing, China | Wang, J., Minzu University of China, Beijing, China | Tian, B., Minzu University of China, Beijing, China | Huang, J., Minzu University of China, Beijing, China | Zhang, R., Minzu University of China, Beijing, China | Lin, Y., Minzu University of China, Beijing, China | Xiao, Z., Minzu University of China, Beijing, China
Tea is one of the most consumed beverages worldwide and the relatively high levels polyphenols is benefit for health. In this study, we developed an efficient system for proliferation of callus from 'Anji Baicha', a cultivar of tea (Camellia sinensis). Callus tissue was initially induced by culturing leaf explants on medium containing different plant growth regulators. For callus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D), alpha-naphthalene acetic acid (NAA), and N6 -benzyladenine (BA). The frequency of callus induction from leaf explants reached 90.21% on 1.0 mgㆍL-¹ TDZ and the developed callus was reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D, indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferation and accumulation of tea polyphenols. The growth index value and tea polyphenol content of callus cultured on MS medium containing 0.5 mgㆍL-¹ TDZ and 1.0 mgㆍL-¹ IAA was maximally 1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as 17.449%. We also measured the antioxidant activity of all samples and the callus with the highest tea polyphenol content also exhibited high potential radical scavenging activity.
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