Detection of Food-Grade Hydrogen Peroxide by HRP-Biocomposite Modified Biosensors
2017
Chang, S.C., Pusan National University, Busan, Republic of Korea
A new amperometric biosensor has been developed for the detection of hydrogen peroxide (H₂O₂). The sensor was fabricated through the one-step deposition of a biocomposite layer onto a glassy carbon electrode at neutral pH. The biocomposite, as a H₂O₂ sensing element, was prepared by the electrochemical deposition of a homogeneous mixture of graphene oxide, aniline, and horseradish peroxidase. The experimental results clearly demonstrated of that the sensor possessed high electrocatalytic activity and responded to H2O2 with a stable and rapid manners. Scanning electron microscopy, cyclic voltammetry, and amperometry were performed to optimize the characteristics of the sensor and to evaluate its sensing chemistry. The sensor exhibited a linear response to H₂O₂ in the range of 10 to 500 microM concentrations, and its detection limit was calculated to be 1.3 microM. The proposed sensing-chemistry strategy and the sensor format were simple, cost-effective, and feasible for analysis of "food-grade H₂O₂" in food samples.
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