Transgenic tomato expressing dsRNA of juvenile hormone acid O-methyl transferase gene of Helicoverpa armigera (Lepidoptera: Noctuidae) affects larval growth and its development
2017
Navale Prakash M., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Manamohan Maligeppagol, Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Asokan R., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Krishna V., Kuvempu University, Shimoga, Karnataka, India | Sharath Chandra G., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Prasad Babu K., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Latha J., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India | Krishna Kumar N.K., Bioversity International - Delhi, NASC Complex, Pusa Campus, New Delhi, India | Ellango R., Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru, India
Helicoverpa armigera is an important pest infesting a number of crops of high commercial value leading to huge economic losses globally. RNA interference (RNAi) is a potent tool for the control of insect pests and towards this objective, transgenic tomato expressing dsRNA of Juvenile hormone acid methyl transferase (JHAMT) gene of H. armigera was developed. The H. armigera larvae fed on HaJHAMT tomato leaves led to a severe (90%) down regulation of the cognate gene expression thus adversely affected the feeding and metamorphosis. Reduction in larval and pupal weight and inability to undergo complete metamorphosis were observed that led to larval-pupal intermediates and subsequently affected the eclosion. Thus, JH down regulation is an attractive target due to specificity to insects and its important role in insect growth and development to engineer insect resistance in crops using RNAi.
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