Plant regeneration from organogenic calli in Mussaenda varieties Doña Eva, Queen Sirikit and Alma Javier
2017
Pamiloza, R.P. | Pateña, L.F. | Caymo, L.S. | Barba, R.C.
Mussaenda is among the economically important ornamental species that are used as potted plants or landscape materials. There are more than 200 species of which some are endemic to the Philippines. Hybridization started in the 1940s at the UPLB [University of the Philippines Los Baños] with then Dean of the College of Agriculture (CA), Dr. Dioscoro L. Umali. The conventional breeding work was continued at the CA's Department of Horticulture and Institute of Plant Breeding with several hybrids being produced. To complement conventional breeding, non-conventional techniques are being done to improve Mussaenda. New genotypes, particularly those difficult to propagate e.g. Doña Eva, can be mass-produced using tissue culture. Three varieties, Mussaenda Doña Eva, Queen Sirikit and Alma Javier, were cultured in vitro. Explants included petaloids, ovarian wall and leaves. Callus induction and proliferation was observed using Murashige and Skoog's medium with BAP, NAA/IAA and myo-inositol after 2 to 3 weeks in culture. Shoots were regenerated from organic calli after 6 to 7 weeks. Adventitious roots were observed after 1 to 2 weeks. Results of the study showed that difficult-to-propagate varieties of Mussaenda can be mass-produced using tissue culture via organogenesis pathway.
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