Biochemical and biological activities of UV treated Peganum harmala seeds
2013
Mahmood, Y.
The plants are one of the rudimentary sources for the necessities of human beings. One consecration from nature is herbs which have been using since prehistoric eras fix health care. Man-made drugs are expensive and unaffordable for approximately two third of human population. Seed vigor and germination are among the problems for the cultivation of medicinal plants and agronomical crops. To address this problem different physico-chemical techniques are used for seed priming. In the current study Peganum hermala seed were germinated after their UV treatment at different time intervals. Seedling were harvested at particular day of germination. UV will work two fold for sterilization as well as mutation in the Peganum hermala seeds. Seedling as well as seed extracts were evaluated by different assays like total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging for their antioxidant activity. The antimicrobial activity will be determined by using disc diffusion assay against selected antifungal and antibacterial strains. Micro dilution method was used to determine the minimum inhibitory concentrations (MIC). The seed extracts were tested for their toxicological potential by hemolytic activity and brine shrimp assay. Statistical analysis of the collected data was carried out by applying regression technique.
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