Gene expression in response to Cotton Leaf Curl Virus infection in Gossypium hirsutum under variable environmental conditions
2017
Rehman, Iqra (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Aftab, Beenish (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Bilal Sarwar, Muhammad (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Rashid, Bushra (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Ali, Qurban (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Majid, Muhammad Umair (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Hassan, Sameera (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Ali, Muhammad Azam (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Nasir, Idrees Ahmad (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Saleem Haider, Muhammad (University of the Punjab, Lahore (Pakistan). Institute of Agricultural Sciences) | Husnain, Tayyab (University of the Punjab Lahore (Pakistan). Centre of Excellence in Molecular Biology)
Cotton Leaf Curl Disease (CLCuD) is one of the threatening constrains of cotton production in Pakistan for which no adequate remedy is available until now. Local variety of Gossypium hirsutum (FH-142) was grown in field and infected naturally by CLCuV under variable range of temperature and humidity. Plants showed thickening of veins in lower leaf surface at 34°C and 60% relative humidity at 15days post infection (dpi) and curling of leaf margins at 33°C with 58% relative humidity at 30dpi. Remarkable leaf darkening was observed with reduced boll formation at 45dpi at 26°C and 41% relative humidity. Enation developed, severe thickening and curling of leaves intensified and plants showed dwarf growth at 60dpi at 24°C with 52% relative humidity. PCR amplification of Rep associated gene confirmed the presence of CLCuD-associated begomovirus in the infected samples. Quantitative RT-PCR confirmed the amplification and differential expression of a number of pathogen stress responsive genes at different levels of temperature and humidity. This observation predicts that Cotton Leaf Curl Virus (CLCuV) interacts with several host genes that are upregulated to make plants susceptible or suppress other genes to overcome host defense responses.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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