Single Nucleotide Polymorphism of TBC1D1 Gene Association with Growth Traits and Serum Clinical-Chemical Traits in Chicken
2018
Prabuddha Manjula, Chungnam National University, Deajeon, Republic of Korea | Cho, S., Chungnam National University, Deajeon, Republic of Korea | Suh, K.J., Chungnam National University, Deajeon, Republic of Korea | Seo, D., Chungnam National University, Deajeon, Republic of Korea | Lee, J.H., Chungnam National University, Deajeon, Republic of Korea
TBC1D1 gene has known functional effects on body energy homeostasis and glucose uptake pathway in skeletal muscle tissue. This biological function is reported to have significant effects on traits of growth and meat quality in chicken. In this study, we focused on two single nucleotide polymorphisms (SNPs) (g.70179137A greater than G and g.70175861T greater than C) identified through SNP annotation information of Korean native chicken and previous literature for TBC1D1 in chicken. Association of SNPs in TBC1D1 with growth and serum clinical-chemical traits were evaluated. A total of 584 male and female birds from five Korean native chicken lines were used in the study. The SNP1 (g.70179137A greater than G) is located in intron 11 and SNP2 (g.70175861T greater than C) is a non-synonymous missense mutation in exon 10, responsible for the amino acid change from Methionine to Valine. The A allele of SNP1 and T allele of SNP2 had the highest allele frequencies. Both SNPs indicated moderate polymorphism information content values (0.25 less than PIC less than 0.5). Association results shown that SNP1 was significantly associated with BW00, BW20, GR14-16 and carcass weight (P less than 0.05). The AA genotype had higher value for all these traits except BW00. Whereas, genotype GG have lower values for BW20, GR14-16, GR18-20 and carcass weight. The sGOT level was significant with SNP1 but not with the SNP2. The GG genotype had the highest sGOT value and AG reported the lowest. BW20 was significantly associated with SNP2 (P less than 0.05). The body weight and carcass weight values of CC and TC genotypes were higher than those of TT genotype.
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