Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
2018
Yuetao Li, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China | Yongkun Zhao, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Cuiling Wang, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China | Xuexing Zheng, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Hualei Wang, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Weiwei Gai, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Hongli Jin, Changchun SR Biological Technology Co., Ltd., Changchun, China | Feihu Yan, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Boning Qiu, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Yuwei Gao, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Nan Li, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Songtao Yang, Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China | Xianzhu Xia, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV pseudovirus-based neutralization assay. This study used the human immunodeficiency virus (HIV) lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were notably similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. This study has successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.
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