Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea
2018
Chon, J.W., Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA | Seo, K.H., Konkuk University, Seoul, Republic of Korea | Bae, D., Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA | Park, J.H., Joy Animal Hospital, Ansan, Republic of Korea | Saeed Khan, Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA | Sung, K., Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the αtoxin gene, but not for the β, ε, or toxin genes; therefore, all strains were identified as type A C. perfringens. All isolates were cpe-negative, whereas the β2 toxin gene was identified in 83.9% and 61.9% of isolates from DD and ND, respectively. Most isolates were susceptible to ampicillin (94%), chloramphenicol (92%), metronidazole (100%), moxifloxacin (96%), and imipenem (100%). However, 25.0% and 21.2% of isolates were resistant to tetracycline and clindamycin, respectively. Molecular subtyping of the isolated strains was performed by using pulsed-field gel electrophoresis. Fifty-two isolates were classified into 48 pulsotypes based on more than 90% similarity of banding patterns. No notable differences were observed among the isolates from DD and ND.
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