Biosensors Using Hybridization Chain Reaction - Design and Signal Amplification Strategies of Hybridization Chain Reaction
2018
Park, C.R., Incheon National University, Incheon, Republic of Korea | Park, S.J., Incheon National University, Incheon, Republic of Korea | Lee, W.G., Incheon National University, Incheon, Republic of Korea | Hwang, B.H., Incheon National University, Incheon, Republic of Korea
Signal amplification strategies are essential for sensitive and efficient detection. Among the recent amplification strategies, the hybridization chain reaction has been intensively studied because it has advantages of reaction at constant temperature and detection at low cost without specialized equipment. In this review, we have discussed how to adjust experimental conditions of the hybridization chain reaction and attractive signal amplification techniques including colorimetric, fluorescence, and electrochemistry. As a result, many studies using the hybridization chain reaction have been successful in detecting sensitive signals by enhancing the signaling of the various targets. These exciting features of the hybridization chain reaction have the potential to be widely used in many areas such as in situ disease diagnosis, food, and environmental analysis. Therefore, various platforms developed by applying this technology are expected to play an essential role as an efficient biosensor in many fields in the near future.
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