Antioxidant and alpha-Glucosidase Inhibitory Activities of the Extracts of Aster koraiensis Leaves
2018
Lee, T.G., Clinical Medicine Division, KIOM, Daejeon, Republic of Korea | Hyun, S.W., Herbal Medicine Research Division, KIOM, Daejeon, Republic of Korea | Lee, I.S., Herbal Medicine Research Division, KIOM, Daejeon, Republic of Korea | Park, B.K., Clinical Medicine Division, KIOM, Daejeon, Republic of Korea | Kim, J.S., Herbal Medicine Research Division, KIOM, Daejeon, Republic of Korea | Kim, C.S., Clinical Medicine Division, KIOM, Daejeon, Republic of Korea
Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via alpha-glucosidase inhibitory assays. The mode of inhibition by AKEs on alpha-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent alpha-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had alpha-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant alpha-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36mg/mℓ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10mg/mℓ. It can therefore be used as a source of therapeutic agents to treat diabetes patients.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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