Glycoproteins isolated from Atractylodes macrocephala Koidz improve protective immune response induction in a mouse model
2018
Kim, K.A., Chonbuk National University, Jeonju, Republic of Korea | Son, Y.O., Gwangju Institute of Science and Technology, Gwangju, Republic of Korea | Kim, S.S., Chonbuk National University, Jeonju, Republic of Korea | Jang, Y.S., Chonbuk National University, Jeonju, Republic of Korea | Baek, Y.H., Chonbuk National University, Jeonju, Republic of Korea | Kim, C.C., Chonbuk National University, Jeonju, Republic of Korea | Lee, J.H., Chonbuk National University, Jeonju, Republic of Korea | Lee, J.C., Chonbuk National University, Jeonju, Republic of Korea
This study examined the efficacy of Atractylodes macrocephala Koidz (AMK) protein and polysaccharide extracts as adjuvant or adjuvant booster when given together with porcine pleuropneumonia vaccine. Experimental mice (n = 5/group) were subcutaneously immunized with 25 ㎍ ApxIIA No.3 antigen, a target protein against A. pleuropneumoniae, together with alum and/or various concentrations (0-500 ㎍) of the AMK extracts, while the control group received PBS only. Immunization with ApxIIA No.3 antigen increased the antigen-specific IgG titer and this increase was enhanced in the immunization together with AMK protein, but not polysaccharide extract. Supplementation of AMK protein extract exhibited dosedependent increases in the antigen-induced protective immunity against A. pleuropneumoniae challenge and in the lymphocyte proliferation specific to the antigen. Glycoproteins present in the AMK extract were the active components responsible for immune response induction. Collectively, the present findings suggest that AMK glycoproteins are useful as immune stimulating adjuvant or adjuvant booster.
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