Determination of molecular diversity with ISSR markers in some cultivated alfalfa (Medicago sativa L.) ecotypes
2016
Ertuş, M. M. | Sabancı, C. O. | Şensoy, S.
The relationships among total 76 ecotypes of alfalfa (Medicago sativa L.), 70 landraces and 6 cultivars, collected in Van and neighboring provinces were investigated in the present study. In the molecular method, Twelve ISSR primers were used and 85 polymorphic bands were obtained. The genetic distances between the ecotypes were expressed by Euclidean coefficients. The genetic variation among alfalfa ecotypes was examined in 16 groups based on the localities, landraces and check cultivars. The highest genetic variations and polymorphisms (H = 0.245, I = 0.366, and 70.59%) were found in Gurpinar localities. As a result, a high genetic diversity was found out among the ecotypes and cultivars of allogamous Medicago sativa L.
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