Molecular characterization of chitinase and β-1, 3-glucanase gene of soybean plant growth promoting bacterium Bacillus sp. SJ-5

Shekhar Jain , Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India; Devendra Kumar Choudhary , Block ‘E-3’, 4th Floor, Amity University Campus, Sector-125, Noida-201313, Gautam Buddha Nagar, UP, India.; Kanti Prakash Sharma , Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India; Sarita Kumari , Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India

Molecular characterization of chitinase and β-1, 3-glucanase gene of soybean plant growth promoting bacterium Bacillus sp. SJ-5

2016

Shekhar Jain (Department of Biosciences, College of Arts, Science & Humanities (CASH), Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India) | Devendra Kumar Choudhary (Amity Institute of Microbial Technology (AIMT), Block ‘E-3’, 4th Floor, Amity University Campus, Sector-125, Noida-201313, Gautam Buddha Nagar, UP, India.) | Kanti Prakash Sharma (Department of Biosciences, College of Arts, Science & Humanities (CASH), Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India) | Sarita Kumari (Department of Biosciences, College of Arts, Science & Humanities (CASH), Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India)

Plant-growth-promoting bacteria (PGPB) colonize the rhizosphere of many plant species and confer beneficial effects, such as increased plant growth and reduced susceptibility to diseases caused by plant pathogenic fungi. The aim of the present study was to molecularly characterize the presence of biocontrol gene chitinase and β-1, 3-glucanase of the Bacillus sp. SJ-5 to understand its role in fungal pathogen inhibition. Genomic DNA was isolated from Bacillus sp. SJ-5 and chitinase and β-1, 3-glucanase gene were amplified using specific primers. On the agarose gel 402 pb and750 bp bands were detected for chitinase and β-1, 3-glucanase respectively. Upon homology analysis it confirms the presence of chitinase and β-1, 3-glucanase gene in SJ-5 and sequences submitted to Genbank. The study indicates the potential of this PGPB to inhibit fungal pathogen through cell wall degrading enzymes production.

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