Жизнеспособность спор Bacillus subtilis в сухой препаративной форме при длительном хранении препарата
2020
Николаев, А. (Academy of Sciences of Moldova. Institute of Genetics, Physiology and Plant Protection, Chisinau (Republic of Moldova) | Максимова, И. | Николаева, C.
The purpose of the research is to create a dry preparative form of a microbiological product that allows producing and storing the preparation for a long time. The objective of the study is to determine the viability of spores in the preparation over various periods of preparation storage. To determine the spore titer, colonies were counted using digital images when sowing various dilutions in samples on the nutritive medium. The article presents data on the viability of Bacillus subtilis spores in a dry preparation, depending on the shelf life of the fungicide. The titer of viable spores in the initial preparation was 9.1*10 9 spores per gram, after 5 months of storage - 8.6*109 spores / 1g, after 36 months - 5.7*109 spores / 1g, after 72 months - 2.7*109 spores / 1g. After 6 years storage of the dry preparative form, the titer of viable spores was comparable with the liquid form of the fungicide currently used and not storable for a long time.
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