Utilization of simple sequence repeats markers in the genetic characterization of Philippine rubber, Hevea brasiliensis (Wild. ex A. Juss) Muell. Arg., germplasm
2015
Cantila, A.Y. | Espino, R.R.C. | Sales, E.K.
Simple Sequence Repeats (SSRs) were utilized to genetically characterize rubber genotypes among 86 clones of rubber germplasm in the Philippines. Twenty-two out of 40 screened SSR primers were found to be informative with a mean of 99.09% polymorphic rate. These primers generated 119 brands with a mean of 5.41 bands per primer in a corresponding range of 100 to 491 base pairs (bp). From 119 alleles, 118 were polymorphic and 76.4 were effective. The resolving power of molecular markers measured as Polymorphism Information Content (PIC) had a mean value of 0.65. SSRs had detected a broad genetic base of 0.30 in Unweighted Pair Group Method with Arithmetic Mean (UPGMA) indicated by Jaccard's similarity coefficient. On the other hand, five ideal groups were computed by Ad hoc statistics for Bayesian grouping as determined using the Structure 2.3.4 program with high replications and iterations of Markov Chain Monte Carlo (MCMC) and admixture model/correlated allele frequencies, respectively. SSRs have provided efficient, reliable and useful derivation of allelic variations, polymorphism information and genetic similarities. The derived ideal groups showed potential use in the decision-making process as to what clones are to be bred.
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