Tocopherol content in cold-pressed oil from different sunflower cultivars grown in Serbia
2018
Grahovac, Nada (https://orcid.org/0000-0002-6635-3568) | Sakač, Zvonimir | Kravić, Snežana (https://orcid.org/0000-0003-4056-2000) | Stojanović, Zorica (https://orcid.org/0000-0001-8892-3157) | Romanić, Ranko (https://orcid.org/0000-0002-5329-4511) | Lužaić, Tanja (https://orcid.org/0000-0003-0424-5972) | Cvejić, Sandra | Jocić, Siniša (https://orcid.org/0000-0002-2189-9372) | Marjanović-Jeromela, Ana (https://orcid.org/0000-0002-7663-0696)
Vegetable oils rich in antioxidant are of great interest in the food industry as they decelerate oxidative degeneration of lipids and therefore promote the quality as well as the nutritional value of food. Sunflower (Helianthus annuus L.) is one of the most abundant annual crops in the world that is grown for edible oil which is a good source of bioactive compounds. Tocopherols belong to the most important bioactive compounds in oil which have many health benefits for human. These compounds preserve oil from peroxidation by scavenging lipid peroxyl radicals. In humans, tocopherols play an important role as vitamin E, contributing to maintaining the immune system and delaying the pathogenesis of a variety of degenerative diseases. Over the last few years, increased interest for natural antioxidant in cold‐pressed plant oils has been observed since they have better nutritive properties in comparation to refined ones. In this work, eleven cold-pressed sunflower oils obtained from sunflower cultivars (cv.) cultivated in Serbia (2017 harvest) were studied for their tocopherols contents. Quantification of tocopherols was carried out using high performance liquid chromatography on a column Nucleosil 100-5 NH2 with fluorescence detection (λex=280 nm, λem=340 nm). The mobile phase was n-hexane/ethyl acetate (70/30, v/v) with a flow rate of 1 mL/min. The relative retention value and maximum values of absorption at the given relative retention time were used to identify and confirm the presence of tocopherols in injection volume of 20 μL solution samples obtained by diluting of oil (300 µL) in n-hexane (2 mL). This research verified the dominant presence of α-tocopherol in cold-pressed sunflower oils. The content of α-tocopherol ranged between 453.93 mg/L oil in cv. Dusko and 716.07 mg/L oil in cv. NS Konstantin. The results have shown that there were differences in tocopherols content among tested cultivars, indicating the great genetic potential for improvement.
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