Prevalence and molecular characterization of tomato spotted wilt virus (TSWV) in pepper growing areas of Mediterranean region
2017
Turkmen, E.
Tomato spotted wilt virus (TSWV), belonging to Tospovirus genus in Bunyaviridae family has been known as only viral agent causing infections in insects and plants. TSWV consists of three genomic single-stranded RNA denoted as S, M, and L. The M RNA responsible for the expression of movement of the virus in host cells with the non-structural protein translated by NSm region which is important for NSM gene-mediated resistance in durability. In this study, it was aimed to determine seasonal prevalence of TSWV on pepper growing areas in the Mediterranean Region. Twenty-six pepper plants from Antalya and İçel provinces were found to be infected by TSWV according to DAS-ELISA and RT-PCR results. Multiple alignment and phylogenetical comparison with homologous isolates retrieved from GenBank was carried out according to the NSm region of the local TSWV isolates. All turkish TSWV isolates showed high nucleotide homology, except one isolate (29) from Antalya. The isolate '29' was genetically different from all others and clustered with the variants of TSWV isolates in GenBank from various countries.
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