Detection of foot and mouth disease virus shedding in milk of apparently healthy buffaloes and cattle of Punjab, Pakistan

Zeeshan Nawaz. Department of Microbiology E-mail:zeeshannawaz@gcuf.edu.pk; Abu Baker Siddique. Department of Microbiology; Muhammad Asif Zahoor. Department of Microbiology; Bilal Aslam. Department of Microbiology; Muhammad Kashif Zahoor. Department of Zoology; Shahid Ali. Department of Microbiology; Sultan Ali. Institute of Microbiology

Detection of foot and mouth disease virus shedding in milk of apparently healthy buffaloes and cattle of Punjab, Pakistan

2019

Zeeshan Nawaz(Government College University, Faisalabad (Pakistan). Department of Microbiology) E-mail:zeeshannawaz@gcuf.edu.pk | Abu Baker Siddique(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Muhammad Asif Zahoor(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Bilal Aslam(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Muhammad Kashif Zahoor(Government College University, Faisalabad (Pakistan). Department of Zoology) | Shahid Ali(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Sultan Ali(University of Agriculture, Faisalabad (Pakistan). Institute of Microbiology)

Foot and mouth disease is among the top listed livestock diseases causing severe economic losses. The aim of the present study was to determine the seroprevalence of FMD and detection of FMD virus shedding in milk of apparently healthy buffaloes and cattle of Pakistan. A total of 30 dairy farms were selected and registered in rural areas of Punjab consisting of minimum 15 animals. A total of 180 serum samples were collected and subjected to Non structural proteins (NSP) ELISA. The milk of sero-positive animals were collected and detected for the presence of FMD virus using reverse transcriptase PCR (RT-PCR). The results of current study showed overall seroprevalence 71.66 percent (129/180) with 65.38 percent in buffaloes and 76.47 percent in cattle. The FMD virus was detected in 24.03 percent (31/129) of sero positive samples. Among the FMD virus positive samples 65.51 percent belongs to serotype O and 35.48 percent belongs to Asia I, while none of the other serotypes were detected. The detection of FMD virus from the milk of apparently healthy buffaloes and cattle is an alarming situation and it may be considered as a potential role in the transmission of FMD.

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