Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis in Murrah buffalo (Bubalus bubalis)

Kamaldeep Dhundwal. Department of Animal Genetics and Breeding; B.L. Pander. Department of Animal Genetics and Breeding; D.S. Dalal. Department of Animal Genetics and Breeding; Ankit Magotra. Department of Animal Genetics and Breeding E-mail:ankitoms@gmail.com; Dinesh Mittal. Department of Veterinary Public Health and Epidemiology; M. Singh. College of Veterinary Science. College Central Laboratory; Anika Malik. Department of Veterinary and Animal Husbandry Extension Education; Asha Garg. Animal Genomic Lab. Department of Animal Genomic Lab

Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis in Murrah buffalo (Bubalus bubalis)

2019

Kamaldeep Dhundwal(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Animal Genetics and Breeding) | B.L. Pander(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Animal Genetics and Breeding) | D.S. Dalal(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Animal Genetics and Breeding) | Ankit Magotra(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Animal Genetics and Breeding) E-mail:ankitoms@gmail.com | Dinesh Mittal(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Veterinary Public Health and Epidemiology) | M. Singh(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). College of Veterinary Science. College Central Laboratory) | Anika Malik(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Department of Veterinary and Animal Husbandry Extension Education) | Asha Garg(Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (India). Animal Genomic Lab. Department of Animal Genomic Lab)

Mannose Binding Lectins (MBL) gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker for milk production traits to control mastitis A total of 60 Murrah buffaloes were selected to characterize exon 2 of MBL1 gene to identify polymorphism and its association with mastitis. A 401 bp PCR fragment of MBL1 gene targeting g.2686T GT C was amplified and digested with HaeIII restriction enzyme. Genotype analysis using PCR-RFLP revealed a monomorphic CC banding pattern. Sequencing was also carried out to explore other SNPs. The result indicates highly conserved DNA sequence in Murrah buffalo. This study provides preliminary information that the targeted region of MBL1 gene in water buffalo has no significant association with mastitis resistance which maybe a breed specific characteristic. Since present study has formulated the results based on a relatively small sample, further studies are required to validate these results in large samples.

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