Comparative study of methodologies to determine the antioxidant capacity of Al-toxified blueberry amended with calcium sulfate
2015
Meriño-Gergichevich, C(Universidad de La Frontera Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO)) | Ondrasek, G(University of Zagreb Faculty of Agriculture Department of Soil Amelioration) | Zovko, M(University of Zagreb Faculty of Agriculture Department of Soil Amelioration) | Šamec, D(Institute Ruđer Bošković Department of Molecular Biology) | Alberdi, M(Universidad de La Frontera Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO) ,Universidad de La Frontera Facultad de Ingeniería y Ciencias Departamento de Ciencias Químicas y Recursos Naturales) | Reyes-Díaz, M(Universidad de La Frontera Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO) ,Universidad de La Frontera Facultad de Ingeniería y Ciencias Departamento de Ciencias Químicas y Recursos Naturales)
Blueberry (Vaccinium corymbosum L.) is well adapted to acidic soils where aluminum (Al3+) can be linked to oxidative stress and antioxidant capacity (AC). Calcium sulfate (CaSO4) is used to alleviate Al3+ because it does not alter soil acidity. However, the role of Ca addition in AC, based on a single electron transfer reaction (SET), remains unknown.The aim was to evaluate the AC using SET methodologies, i.e., 2,2-diphenyl-2-picrylhydrazyl (DPPH), 2,2´-Azinobis-bis (3 ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing-antioxidant power (FRAP). Blueberry cultivars Elliot and Jersey were grown and exposed to the following treatments for 15 days: Control (nutrient solution alone, pH 4.5); control+Al (Al); control+Al+Ca (Al+Ca) and control+Ca (Ca). The Ca and Al concentrations, total phenol (TP) content and radical scavenging activity (RSA) were evaluated at 7 and 15 days. The Al+Ca and Ca treatments increased the Ca concentration in the leaves (22%) and roots (40%) of both cultivars compared with the control. The Ca in the tissues varied with cultivar and time. After 15 days, increases in TP, DPPH, ABTS and FRAP were detected. The cultivars showed positive relationships between the TP and AC in the leaves and roots. Thus, CaSO4 is an important tool to improve the AC in Al-stressed fruit crops grown in acid soils.
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