Analysis of plant growth promoting potential of endophytes isolated from Echinacea purpurea and Lonicera japonica
2016
Gupta, H(Shoolini University of Biotechnology and Management Sciences Department of Biotechnology) | Saini, R. V(Shoolini University of Biotechnology and Management Sciences Department of Biotechnology) | Pagadala, V(CSIR-Institute of Microbial Technology) | Kumar, N(University of North Carolina Eshelman School of Pharmacy Department of Chemical Biology and Medicinal Chemistry) | Sharma, D. K(University of North Carolina Eshelman School of Pharmacy Department of Chemical Biology and Medicinal Chemistry) | Saini, A. K(Shoolini University of Biotechnology and Management Sciences Department of Biotechnology)
Plant endophytes help in maintaining plant health by means of their biofertilizer and biocontrol attributes and, are currently being explored for their ability to produce novel biologically active compounds. Herein, we have isolated beneficial endophytic bacteria from Echinacea purpurea (EF.B3) and Lonicera japonica (LS.B11) that showed phosphate solubilization, siderophore, indole acetic acid and hydrogen cyanide production, and fixation of atmospheric nitrogen. Additionally, the endophytes also conferred antifungal activity against Fusarium sp., Rhizoctonia sp., Pythium sp. and Alternaria sp. When tested in plantae, the LS.B11 and EF.B3 strains were able to promote plant growth and control fungal infections in peaseedlings. Both strains were found to be endophytic as tested by RAPD and viability count. Based on 16S rRNA gene sequencing, we show that the LS.B11 and EF.B3 strains are related to Pseudomonas sp. And Burkholderia sp. By using degenerate primers, we identified genes related to polyketide synthases and non-ribosomal peptide synthetases in EF.B3 and LS.B11, respectively that are typically involved in the production of antimicrobial compounds. Therefore, we conclude that both endophytes can be used for increasing agriculture productivity and in the production of antimicrobial compounds for crop improvement.
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