Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor
2018
Schwartz, Mathieu | Perrot, Thomas | Aubert, Emmanuel | Dumarçay, Stéphane | Favier, Frédérique | Gérardin, Philippe | MOREL-ROUHIER, Melanie | Mulliert, Guillermo | Saiag, Fanny | Didierjean, Claude | Gelhaye, Éric
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants.Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level.Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones.Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site.These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique
