Identification of Echinococcus granulosus strains using polymerase chain reaction-restriction fragment length polymorphism amongst livestock in Moroto district, Uganda

Chamai, Martin; Omadang, Leonard; Erume, Jospeh; Ocaido, Michael; Oba, Peter; Othieno, Emmanuel; Bonaventure, Straton; Kitibwa, Annah

Identification of Echinococcus granulosus strains using polymerase chain reaction-restriction fragment length polymorphism amongst livestock in Moroto district, Uganda

2016

Chamai, Martin(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Omadang, Leonard(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Erume, Jospeh(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Ocaido, Michael(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Oba, Peter(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Othieno, Emmanuel(Makerere University College of Health Sciences Department of Pathology) | Bonaventure, Straton(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences) | Kitibwa, Annah(Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity Department of Biomolecular Resources and Biolaboratory Sciences)

A descriptive study was conducted to identify the different strains of Echinococcus granulosus occurring in livestock in Moroto district, Uganda.Echinococcus cysts from 104 domestic animals, including cattle, sheep, goats and camels, were taken and examined by microscopy, polymerase chain reaction with restriction fragment length polymorphism and Sanger DNA sequencing. Echinococcus granulosus genotypes or strains were identified through use of Bioinformatics tools: BioEdit, BLAST and MEGA6. The major finding of this study was the existence of a limited number of E. granulosusgenotypes from cattle, goats, sheep and camels. The most predominant genotype was G1 (96.05%), corresponding to the common sheep strain. To a limited extent (3.95%), the study revealed the existence of Echinococcus canadensis G6/7 in three (n = 3) of the E. granulosus-positive samples. No other strains of E. granulosus were identified. It was concluded that the common sheep strain of Echinococcus sensu stricto and G6/7 of E. canadensiswere responsible for echinococcal disease in Moroto district, Uganda.

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