Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique

Siti Shapor Siraj; Chan, S. C.; Tan, S. G.; Khatijah Yusoff

Rapid and non-radioactive detection method of microsatellites in Mystus nemurus: a refined technique

2005

Siti Shapor Siraj | Chan, S. C. | Tan, S. G. | Khatijah Yusoff

A simple and rapid method of DNA microsatellite isolation based on the Random Amplified Microsatellites(RAMs) PCR technique was used in this study. The work presented here is part of a continuous effort in refiningand perfecting the technique for more rapid, effective and optimum productivity in single locus microsatellitemarker development for the River catfish, Mystus nemurus. The current refined protocol for microsatelliteisolation was able to detect a total of 135 microsatellite regions resulting in 42 unique genomic sequences beingsubmitted to GenBank. This refined technique is able to reduce the total time required from peR cloning tillsequencing specific microsatellite regions to less than three and a half months.

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