Complete genome-based approach and substrate change analysis on Lactobacillus fermentum L1 producing high-yield conjugated linoleic acid
2020
Ji, J. J. | Qian, B. P. | Yang, Z. B. | Huang, A. X. | Ma, Q. W.
A total of 300 strains of lactic acid bacteria (LAB) were screened for conjugated linoleic acid (CLA) production. The major isomers of CLA in the fermentation broth of the strain with the highest CLA production were analysed by gas chromatography; and whole-genome sequencing analysis was performed to explore the reasons for CLA production. A total of 65 strains of CLA-producing bacteria belonging to four species of LAB were found. The Lactobacillus fermentum L1 with the highest CLA production (1002.486 × 10-³ mg/mL) was isolated from the fermented dough. The gene number of L. fermentum L1 was 2034, and the GC content in the gene region was 53.18%. Moreover, there were high numbers of genes involved in the function of metabolism. In addition, there were two major isomers of CLA (C18:2 10t,12c and C18:2 9c,11t) in the fermentation broth of L. fermentum L1. The change in C:18 fatty acids and the linoleic acid metabolism pathway of L. fermentum L1 revealed that C18:2n6c was the substrate of CLA. The present work provides a scientific basis for the application of CLA-producing with LAB.
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