Pathogenic status, antibiogram, adhesive characteristics, heavy metal tolerance and incidence of integrons of infected fish isolated Aeromonas sp.

Sabayasaohi, P.; Shankar, R. S.; Kumar, D. B.; Kumar, S. P.; Pasim, R. K.

Pathogenic status, antibiogram, adhesive characteristics, heavy metal tolerance and incidence of integrons of infected fish isolated Aeromonas sp.

2019

Sabayasaohi, P. | Shankar, R. S. | Kumar, D. B. | Kumar, S. P. | Pasim, R. K.

Motile aeromonad infection is a common disease in freshwater fish that is difficult to control. This study assessed the pathogenic properties of Aeromonas spp. isolated from major carps at Indian farms. Five selected virulent isolates were screened for antibiogram, biofilm-producing potential, heavy metal tolerance and the presence of integrons. A. sobria, along with two isolates each of A. hydrophila and A. veronii that were found to be pathogenic in an in vivo challenge study, were observed to be resistant to 12 types of antibiotics. However, they did show susceptivity to fluoroquinolones and aminoglycosides. Norfloxacin, chloramphenicol, ciprofloxacin, levofloxacin, amikacin and gentamicin effectively inhibited the growth of all five Aeromonas isolates. The isolates were tolerant to Cu2+ but not Hg2+ in the heavy metal assay. A. sobria and both A. Hydrophila isolates were found to be tolerant to Cr6+. The A. sobria and one A. hydrophila isolate (ATCC 0637P) were strong producers of biofilm. The presence of amplicons for integrons I and II were detected in A.sobria and both A. hydrophila isolates. This investigation produced a baseline information on the prevalence of potential pathogenic Aeromonas in aquaculture systems that could pose a serious risk if they were passed to animals and humans through close contact.

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