Phytochemical screening, phenolic content and antioxidant activity of wild date palm (Phoenix sylvestris Roxb.) fruit extracted with different solvents
2017
Saha, S. | Barua, B. | Sikdar, D.
Fruits are known to have different levels of antioxidant capacity due to the presence of multiple types of phenolic compounds in varying amounts. In this study phytochemical constituents, phenolic content, flavonoid content, and antioxidant activity of Phoenix sylvestris fruit were investigated. Three solvents (methanol, ethanol, and acetone) with different concentrations (50%, 70% and 100%) and water were used for extraction. Phytochemical analyses showed the presence of tanins, flavonoids and saponins. Fruits extracted with 70% acetone showed highest total phenolic content (1554.85 mg GAE/100 g FW) and total flavonoid content (2121.77 mg CEQ/100 g FW) whereas lowest total phenolic content (212.94 mg GAE/100 g FW) and lowest total flavonoid content (130.41 mg CEQ/100 g FW) were found in H2 O extract. Antioxidant activities of different solvent extracts were evaluated using ferric reducing antioxidant power (FRAP) assay, total antioxidant capacity assay, reducing power assay and 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical-scavenging assays. With all the assay methods, 70% acetone extract showed the highest antioxidant capacity. It was also observed that the phenolic content were strongly correlated with the antioxidant activity.
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