Mass propagation of Sungkai (Peronema canescens) for plantation forestry, using tissue culture methods
2004
Rosilah A. A. | Kandasamy K. I.
Sungkai (Peronema canescens) is an indigenous, fast-growing, semi hard-wood species. It is an attractive species for establishing forest plantation models, especially due to its fast growing nature and desirable wood quality, e.g. its whitecoloured wood (popular amongst chop-stick makers). Since, the availability of highquality planting materials are a pre-requisite for any forest plantation programmes, thus, it was necessary to develop an efficient mass propagation method for this species. Cuttings of selected trees (from Sabah) were established in FRIM's nursery, and the resulting plants were used as the explant source for micropropagation experiments. Clean cultures were established using suiface sterilised nodal cuttings. Resulting clean shoot cultures were used for the development of a suitable shoot-multiplication media formulation, including different basal medium types and various concentration and combinations of growth regulators. The incorporation of 0.5mg/L BAP in MS medium was found to be most effective for the induction of shoot multiplication. A sub-culturing cycle of six (6) weeks were adopted for the purpose of mass propagation of these species. MS medium supplemented with 0.1 mg/L BAP was sufficient to induce shoot elongation, thus, producing well-formed shoot cultures, which could then be used either for further multiplication or rooting activities. Shoot cultures rooted well within four (4) weeks upon being cultured on MS medium supplemented with 1mg/L IBA. Complete plantlets were successfully weaned off in our own "Award-wining Weaning Chamber" in our greenhouses. With this system, were have been able to produce over 10,000 plants for our customers within the last six (6) months.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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