Identification of the target corm meristematic tissue from shoot proliferation of banana cultivars
2000
Che Radziah C. M. Z. | Sreeramanan S. | Puspita Deswina | Marziah M.
The banana is a large perennial herbaceous plant. It consists of an underground stem known as corm. Corm tissues are amenable to genetic transformation because they contain a largeı percentage of actively dividing regenerable cells. Berangan was identified to have the greatest potential for commercial cultivation and the elite Berangan selection was known as Intan. In this experiment, Berangan, Intan and Mas, the popular local dessert cultivars, were used. The aim of this study was to identify the target corm tissues which produced the highest number of shoots in 4 weeks. Corm slices of 2mm thick and 5-8mm diameter, numbered I to 5 were cultured onto Murashige & Skoog (1962) medium containing 5 mg/l BAP. Cultures grownı under a 24 hour photoperiod, were provided with white fluorescent at 27°C. Corm tissues started to swell after I week of culture and were transferred to fresh medium after 2 weeks. The shoots were formed and the average number of shoots produced per corm slice was always highest in slice number 2 , followed by number 3. The maximum number of shoots per corm slice obtained were 12 shoots in all cultivars examined, Intan , Berangan and Mas. Corm slice number 2 obtained from this study could be a potential source for establishing transformation protocols for our future investigations.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Universiti Putra Malaysia