Increased of hypoglycemic effect and pancreatic regeneration of Pandanus amaryfollius leaves ethyl acetate extract in streptozotocin-induced diabetic rats

The number of diabetes mellitus patients is continuously growing. Diabetes is a chronic disease and/or a major endocrine disorder caused by a deficiency of insulin production by pancreas or low insulin productivity. Hypoglycemic potentials have been widely examined from plant ingredients to substitute diabetes medicines and treatments. The objective of this research was to investigate a hypoglycemic activity and pancreatic cell regeneration of ethyl acetate extract from Pandanus amaryfollius leaves in streptozotocin-induced diabetic rats. The ethyl acetate extract of pandan leaves was prepared by crushing, macerating, filtrating and evaporating the leaves. Forty rats were grouped into five groups: (1) normal controlled rats, (2) diabetic controlled rats, (3) diabetic rats with feed standard and 0.985 mg/20g diet ethyl acetate extract(K1), (4) diabetic rats with feed standard and 1.97 mg/20g diet ethyl acetate extract (K2),and (5) diabetic rats with feed standard and 2.955 mg/20g diet ethyl acetate extract (K3). The treatments were being conducted for 28 days; blood sampling was taken every 7 days to be analyzed its blood glucose levels, and the final research stage was to analyze its blood insulin levels and MDA levels in the rats’ liver and pancreatic histology. The result showed that the intake of ethyl acetate extract in the diabetic rats could lower their blood glucose levels. The higher the dose of ethyl acetate extract resulted in the lower blood glucose levels, the higherblood insulin levels, and the lower liver damage due to the oxidative stress. In addition, the higher the dose of ethyl acetate extract resulted in the lower insulin resistance in the islet of Langerhans, indicated the smaller the volume of pancreatic β-cells. However, the insulinresistance decreased and the insulin secretion increased; hypoglycemic ability of ethyl acetate extract of fragrant pandan leaves remained high.