Optimization of enzymatic hydrolysis of shortfin scad (Decapterus macrosoma) myofibrillar protein with antioxidant effect using alcalase

Nabilah Husna Ishak; Kang, P. Y.; Norizah Mhd Sarbon

Optimization of enzymatic hydrolysis of shortfin scad (Decapterus macrosoma) myofibrillar protein with antioxidant effect using alcalase

2018

Nabilah Husna Ishak | Kang, P. Y. | Norizah Mhd Sarbon

By employing response surface methodology (RSM), this study aimed to describe conditions that optimized both yield and antioxidant activity for the hydrolysate of shortfin scad (Decapterus macrosoma) myofibrillar protein (SSMH). The study used a three level, factor-face centered, central composite design (CCD) to assess effects from four independent variables; namely, time (60 mins, 120 mins, 180 mins); temperature (45, 55 and 65°C); pH (8, 9, 10); and enzyme/ substrate ratio (1, 2 and 3%). The hydrolysate products were then evaluated for (i) the yield; and (ii) the level of DPPH radical scavenging activity. Optimized hydrolysis conditions were established at 180 mins / temperature 59.49°C / pH 9.93 / and 1% enzyme concentration.Predicted response values (yield: 13.56% and DPPH antioxidant activity: 59.20%) were closeto the experimentally validated values of 13.18 and 56.10%, respectively. Optimized SSMH demonstrated a DH of 40.56% along with higher chelating effects on ferrous ion (90.91%) (IC50 = 0.38 mg/ml) as well as greater hydroxyl radical scavenging activity (96.12%) (IC50 = 1.37 mg/ml). However, lower reducing activity (54.00%) (IC50 = 4.59 mg/ml) and DPPH radical scavenging activity (69.98%) (IC50 = 1.89 mg/ml) were observed. The higher chelating and radical scavenging activity values suggest that SSMH has potential characteristics as a natural antioxidant.

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