Firmicutes is the predominant bacteria in tempeh

Kurosawa, N.; Suwanto, A.; Wahyudi, A.; Rusmana, I.; Radita, R.

Firmicutes is the predominant bacteria in tempeh

2018

Kurosawa, N. | Suwanto, A. | Wahyudi, A. | Rusmana, I. | Radita, R.

Tempeh is an Indonesian fermented soybean inoculated with Rhizopus spp. A number ofculturable bacteria in tempeh had been documented. However, comprehensive study of bacteria population in tempeh has not been reported. This research aimed to analyze species of bacteria in tempeh employing culturable method and metagenomics analysis based on 16S rRNA gene sequences and high-throughput sequencing. Samples were obtained from two tempeh producers in Bogor, Indonesia, i.e. EMP and WJB. Metagenomics analysis indicated that Firmicutes was the predominant phylum in both samples, with Lactobacilalles as the predominant order. Thesecond majority phylum was Proteobacteria. Similarly, the results obtained from culturable technique also showed that Firmicutes was the predominant phylum. One-time boiling of soybean employed for EMP tempeh harbored the highest bacterial diversity than two-times boiling soybean (WJB tempeh). Four orders were the predominant bacteria in EMP tempeh, i.e. Lactobacillales, Clostridiales, Enterobacteriales, and Sphingomonadales, while Lactobacillales and Rhodospirilalles were the only predominant bacteria orders in WJB tempeh.

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