Identity of unregistered rice variety revealed through DNA fingerprinting
2019
Caguiat, J.D. | Enriquez, J.O.S. | Caquiat, X.G. | Cantilla, A. | Abdula, S.E.
Unregistered rice varieties (URV) have been observed in field and used by farmers over a period of time due to its unique and outstanding traits. Characterization of these URV is important in order to determine its precise identify. This study aims to analyze the genetic identify of unregistered rice varieties collected across rice growing environments. A total of 304 URV including ten famous released varieties as checks (IR64, PSB Rc10, PSB Rc82, NSIC Rc216, NSIC Rc218, NSIC Rc222, NSIC Rc224, NSIC Rc240, NSIC Rc302, NSIC Rc308) were genotyped using 7k Infinium SNP distributed across the whole rice genome. Low levels of genetic diversity were observed between the lines ranging from 0-0.578 with an average of 0.167 which coincides with the results for marker diversity. Relationship coefficients of the lines ranged from 0.422-1.000 with an average of 0.83 indicating high levels of relatedness between entry. Close relationship among inbred checks was also observed. Overall, 181 out 294 URV had at least 90% similarity coefficient with the inbred check varieties. A total of 47 lines have at least 90% similarity coefficient with NSIC Rc222, 26 entries with NSIC Rc218, 168 lines with PSB Rc 10, and 15 lines with IR64. Genotypic identify of URV and similarity with the famous inbred varieties was established. This is helpful in determining proliferated seeds' identify and authenticity thereby farmers to have the best quality varieties.
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